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Part II – Molecular Biology and Genetics

Chapter 7 – Principles of Cardiovascular Molecular Biology and Genetics

Elizabeth G. Nabel

  PRINCIPLES OF CELL BIOLOGY AND THE CELL CYCLE, 57
  THE GENETIC CODE: DNA, RNA, AND PROTEIN, 59
  DNA, 59
  RNA, 60
  From Genes to Proteins, 60

  PRINCIPLES AND TECHNIQUES OF MOLECULAR BIOLOGY, 61
  Cloning DNA, 62
  Blotting Techniques, 62
  Polymerase Chain Reaction, 63

  PRINCIPLES OF MOLECULAR GENETICS, 63
  Genotype and the Identification of Disease-Causing Genes, 63
  Genomics, 65
  Proteomics, 66

  GENETIC MODIFICATION OF MICE TO STUDY HUMAN CARDIOVASCULAR DISEASE, 66
  Transgenic Mice, 67
  Gene Inactivation or Knockout Approaches, 67
  Conditional Knockout Mice, 67
  Studies of Mouse Physiology, 68

  GENE TRANSFER, 68
  Vectors, 68

  FUTURE DIRECTIONS, 69
  REFERENCES, 69

Molecular biology and genetics furnish the scientific underpinnings of cardiovascular medicine. Recent discoveries of the genetic bases and molecular pathways of cardiovascular disease have been extraordinary. We are learning about the pathophysiology …