//LABORATORY INVESTIGATION FOR BLOOD AND TISSUE PARASITES 4-u1.0-B978-1-4160-4470-3..50094-X..DOCPDF Clinical Laboratory Diagnosis 4-u1.0-B978-1-4160-4470-3..50094-X--cesec39 4-u1.0-B978-1-4160-4470-3..50094-X--cesec40 4-u1.0-B978-1-4160-4470-3..50094-X--cesec39 fb-non-chapter Appendix I section 4-u1.0-B978-1-4160-4470-3..50094-X--cesec45 LABORATORY INVESTIGATION FOR BLOOD AND TISSUE PARASITES [refer to text] 4-u1.0-B978-1-4160-4470-3..50094-X--cesec40 [refer to text] rearpart 4 section 4-u1.0-B978-1-4160-4470-3..50094-X--cesec39 4-u1.0-B978-1-4160-4470-3..50094-X--cesec40 Staining of blood films 4-u1.0-B978-1-4160-4470-3..X5001-0--rearpart1 text/html; charset=ISO-8859-1 Staining of blood films Clinical Laboratory Diagnosis 4-u1.0-B978-1-4160-4470-3..50094-X bookContent Staining of blood films 4-u1.0-B978-1-4160-4470-3..X5001-0--rearpart1 4-u1.0-B978-1-4160-4470-3..50094-X 4-u1.0-B978-1-4160-4470-3..50094-X 4-u1.0-B978-1-4160-4470-3..50094-X--cesec29 5 Appendix I LABORATORY INVESTIGATION FOR BLOOD AND TISSUE PARASITES 4-u1.0-B978-1-4160-4470-3..50094-X--cesec40 Appendix I Manson's Tropical Diseases 978-1-4160-4470-3 Cook 22nd
LABORATORY INVESTIGATION FOR BLOOD AND TISSUE PARASITESThe blood or tissue parasites are transmitted during vector insect blood feeds and usually continue their life cycle in the human host. The common blood or tissue parasites found belong to the genus of Plasmodium (Table I.2), Trypanosoma (African and South American trypanosomes), Babesia and the Leishmania complex and a few blood spirochaetes.
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Blood parasites can be identified from peripheral blood, bone marrow aspirate, splenic aspirate and gland aspirate, or from tissue biopsy material (Figures I.11–I.14).
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Routine procedures for identification include:
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| Direct staining of blood or impression smears
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| Concentration methods
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| Culture of appropriate sample
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| Detection of parasite DNA using PCR[8]
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| Immunochromatographic rapid tests for the detection of parasite antigen.[9–11]
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Staining of blood filmsGiemsa stainGiemsa stain is a Romanowsky stain and stains chromatin material red and cytoplasm blue. Inclusion bodies within the cell stain red or blue …
