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Chapter 3 – Basic haematological techniques

Carol Briggs,
Barbara J. Bain


Chapter contents
  Haemoglobinometry 24
  Measurement of haemoglobin concentration using a spectrometer (spectrophotometer) or photoelectric colorimeter 24
  Haemiglobincyanide (cyanmethaemoglobin) method 25
  Diluent 25
  Haemiglobincyanide reference standard 25
  Method 26
  Calculation of haemoglobin concentration 26

  Oxyhaemoglobin method 27
  Method 27
  Standard 27

  Direct spectrometry 27
  Direct reading portable haemoglobinometers 28
  Colour comparators 28
  Portable haemoglobinometers 28
  Non-invasive screening tests 28

  Range of haemoglobin concentration in health 28
  Packed cell volume or haematocrit 28
  Microhaematocrit method 29
  International council for standardization in haematology reference method 30
  Surrogate reference method 30
  Range of packed cell volume in health 30

  Manual cell counts and red cell indices 30
  Range of MCHC in health 30

  Manual differential leucocyte count 30
  Method 31

  Basophil and eosinophil counts 32
  Range of eosinophil count in health 32